Objective: Parkinson's disease (PD) is characterized by deterioration of the nigrostriatal system and associated with chronic neuroinflammation. Glial activation has been associated with regulating the survival of dopaminergic neurons and is thought to contribute to PD through the release of proinflammatory and neurotoxic factors, such as reactive nitric oxide (NO) that triggers or exacerbates neurodegeneration in PD. Polyunsaturated fatty acids (PUFAs) exert protective effects, including antiinflammatory, antiapoptotic, and antioxidant activity, and may be promising for delaying or preventing PD by attenuating neuroinflammation and preserving dopaminergic neurons. The present study investigated the effects of fish oil supplementation that was rich in PUFAs on dopaminergic neuron loss, the density of inducible nitric oxide synthase (iNOS)-immunoreactive cells, and microglia and astrocyte reactivity in the substantia nigra pars compacta (SNpc) and striatal dopaminergic fibers.
Methods: The animals were supplemented with fish oil for 50 days and subjected to unilateral intrastriatal 6-hydroxydopamine (6-OHDA)-induced lesions as a model of PD.
Results: Fish oil mitigated the loss of SNpc neurons and nerve terminals in the striatum that was caused by 6-OHDA. This protective effect was associated with reductions of the density of iNOS-immunoreactive cells and microglia and astrocyte reactivity.
Discussion: These results suggest that the antioxidant and antiinflammatory properties of fish oil supplementation are closely related to a decrease in dopaminergic damage that is caused by the 6-OHDA model of PD. 相似文献
This study used RNA interference (RNAi) to explore the effect of NO and inducible nitric oxide synthase (iNOS) on apoptosis and proliferation in the tongue squamous carcinoma cell line Tca8113. Tca8113 cells were transfected with the plasmid pGenesil-1, which expresses iNOS short hairpin RNA (shRNA), or the negative control plasmid pSilencer-HK, and the transfected cells were compared with untransfected cells. The expression of iNOS was detected by histochemistry, and apoptosis was detected by flow cytometry. The expression of iNOS was significantly lower in the pSilencer-iNOS group than in the pSilencer-HK and empty control groups. The apoptosis rate was significantly higher in the pSilencer-iNOS group than in the pSilencer-HK and empty control groups. Growth monitoring showed that proliferation was also inhibited in cells transfected with pSilencer-iNOS. RNAi gene silencing decreased iNOS gene expression, induced apoptosis, and suppressed proliferation in Tca8113 cells. 相似文献
A reflection-type electrochemical cell was used to perform electrochemical in situ X-ray diffraction studies of RuO2 and IrO2 electrodes. X-ray diffractograms were recorded in situ as a function of the polarization time while the electrodes were poised at ?0.5 V vs SCE in 1 M H2SO4 at room temperature. At this potential, strong hydrogen evolution is observed at the surface of the electrodes. In both cases, there is a shift of the X-ray diffraction peaks of the rutile structure towards the lower 2θ angle values with the polarization time, indicating that the volume of the unit cell increases. In the case of RuO2, the unit cell volume increases from 64.00 to 65.34 Å3, which corresponds to a volume increase of ~2%. In comparison, the unit cell volume of IrO2 increases from 65.56 to 66.00 Å3 (0.66%). These changes in the structure of the oxides occur on the time scale of a few hours. These modifications are not totally reversible, as the volume of the unit cell of both RuO2 and IrO2 stays slightly expanded compared to that of the pristine materials when the electrodes are brought back to open circuit conditions after extensive cathodic polarization. In the case of RuO2, the expansion of the unit cell occurs almost exclusively along the a–a plane, suggesting that hydrogen is inserted between the O–O atoms in this plane. The significance of these results in the context of the activation process of RuO2 and IrO2, that is the propensity of these oxides to become better performing electrode materials when they are cathodically polarized, is discussed. 相似文献
BackgroundHundreds of adipokines have been identified, and their extensive range of endocrine functions—regulating distant organs such as oral tissues—and local autocrine/paracrine roles have been studied. In dentistry, however, adipokines are poorly known proteins in the dental pulp; few of them have been studied despite their large number. This study reviews recent advances in the investigation of dental-pulp adipokines, with an emphasis on their roles in inflammatory processes and their potential therapeutic applications.HighlightsThe most recently identified adipokines in dental pulp include leptin, adiponectin, resistin, ghrelin, oncostatin, chemerin, and visfatin. They have numerous physiological and pathological functions in the pulp tissue: they are closely related to pulp inflammatory mechanisms and actively participate in cell differentiation, mineralization, angiogenesis, and immune-system modulation.ConclusionAdipokines have potential clinical applications in regenerative endodontics and as biomarkers or targets for the pharmacological management of inflammatory and degenerative processes in dental pulp. A promising direction for the development of new therapies may be the use of agonists/antagonists to modulate the expression of the most studied adipokines. 相似文献